Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques
CNRS UMR 8601 • Université Paris Descartes
45 Rue des Saints Pères
75270 Paris Cedex 06 • France
Phone: +33 1 76 53 42 14‬
Room R171

Biography

Nicolas Giraud graduated in chemistry from the Ecole Normale Supérieure (Lyon). His early interest for nuclear magnetic resonance stemmed from his internship carried out in 2000 in the group of Prof. Horst Kessler at Technical University of Munich (Germany), on the structural analysis of synthetic peptides. He completed a PhD on Biological NMR at Université Claude Bernard (Lyon) in 2005, in the groups of Prof. Lyndon Emsley, Dr. Martin Blackledge, and Dr. François Penin. In 2006, he moved to the Université Paris Sud (now Université Paris Saclay) where he was appointed Associate Professor in the group led by Prof. Jacques Courtieu. He has worked on the development of high-resolution NMR techniques mainly in the field of chiral analysis and supramolecular interactions. During this period, he has been teaching analytical chemistry at Institut Universitaire de Technologie d’Orsay. In 2013, he became coordinator of the analytical chemistry facility in the Chemistry Department.

    He moved to Université de Paris (Paris Descartes) and became Professor of Chemistry in September 2017. He is head of the research group NMR of Biological Substances, and coordinator of the research team Bio-Spectroscopy (together with Prof. Claire Mangeney). His research interests include the study of challenging molecular or supramolecular systems in the field of Health Sciences, with a particular emphasis on the development of analytical methods targeting their dynamic and structural features. He is teaching chemistry to undergraduate and graduate students in the Department of Fundamental and Biomedical Sciences (Faculty of Sciences). In 2019, he became Chair of the first year of the Master Program Frontiers in Chemistry (together with Dr. Aurélie Perrier-Pineau). 

    In 2011, he received a Young Investigator Award from the French National Research Agency (ANR-2011-JS08-009-01). Over the last years, he has been coordinator or PI in other granted projects, both for research and educational purposes (ANR-13-BS07-0007-03 ;  ANR-11-IDEX-0003). Since 2019, he is coordinator of the project BioDNP funded by the IDEX « Université de Paris 2019 » for young group leaders (ANR-18-IDEX-0001 – call for projects Dynamic Research). He has contributed to organizing several national and international conferences in the field of magnetic resonance, as well as thematic schools. He was member of the organizing committee of the Alpine Conference on Solid-State NMR (now Alpine Conference on Magnetic Resonance in Solids) from 2007 to 2017, and the elected president of the executive board of the GERM (French Group for the Study of Magnetic Resonance) from 2016 to 2018.

 

Curriculum Vitae

  • Since 2017     Professor of Chemistry (section 31) – Université de Paris
    Head, research group on NMR of Biological Substances, UMR 8601
    Coordinator, Team Bio-Spectroscopies (NMR, EPR, Nano-spectroscopies)
    Elected member of the laboratory council of the research unit UMR 8601
    Program Co-chair, 1st year of the Master Frontiers in Chemistry
     
  • 2006 – 2017   Associate Professor (HDR) – Université Paris-Saclay
    Research activity on NMR in Oriented Media, ICMMO, UMR CNRS 8182
    Habilitation (HDR – accreditation to supervise research, Université Paris-Sud, 2014)
    Teachings in analytical chemistry, Institut Universitaire de Technologie d’Orsay
    Coordinator of the analytical chemistry facility in the Chemistry Department, IUT Orsay.
     
  • 2005 – 2006   Agrégé Préparateur – Ecole Normale Supérieure de Lyon
    Chemistry teachings, Préparation à l’Agrégation de Chimie
    Postdoctoral research associate working on Solid-State NMR of Proteins, Laboratoire de Chimie – UMR CNRS – ENSL 5182

     

  • 2002 – 2005   PhD Student – University Claude Bernard Lyon 1
    PhD in Biological NMR defended in 2005 (with highest distinction): Methodological Developments for The Structural and Dynamic Analysis of Proteins by Solid-State Nuclear Magnetic Resonance
    Teaching assistant (moniteur) in chemistry, Ecole Normale Supérieure de Lyon
     
  • 1998 – 2002   Elève Normalien – Ecole Normale Supérieure de Lyon
    Bachelor’s degree (major: physical chemistry, 1999)
    Agregation in chemistry (french highest teaching competitive examination, 2001)
    Master’s degree in molecular and structural physical chemistry, Université Joseph Fourier, Grenoble (2002).

 Keywords 

Analytical chemistry • ultrahigh-resolution NMR • solid-state NMR • paramagnetic NMR • NMR in chiral liquid crystals • methodological development • nuclear spin relaxation • exchange spectroscopy • complex mixtures • enantiomers • metabolites • proteins • dynamic and structural analysis • quantitative analysis • supramolecular interactions

 

Teaching Activities 

  • Lectures, tutorials and practical works in nuclear magnetic resonance (Master Frontiers in Chemistry)
  • Lectures and tutorials in electrochemistry (Licence in Chemistry)
  • Tutorials in chemistry (PACES, 1st year of medical and health studies)

Publications

2020

MnI Complex Redox Potential Tunability by Remote Lewis Acid Interaction
Srinivasan, A. Campos, J., Giraud, N., Robert M. and Rivada-Wheelaghan O.* Dalton Trans., Advance Article (2020https://dx.doi.org/10.1039/D0DT02467H

Multinuclear NMR in Polypeptide Liquid Crystals: Three Fertile Decades of Methodological Developments and Analytical Challenges. Lesot, P., Aroulanda, C., Berdagué, P., Meddour, A., J., Merlet, D., Farjon, J., Giraud, N., & Lafon, O. Progress in Nuclear Magnetic Resonance Spectroscopy, 116, 85-154 (2020) https://dx.doi.org/10.1016/j.pnmrs.2019.10.001

2019

Monitoring Conformational Changes in an Enzyme Conversion Inhibitor Using Pure Shift Exchange NMR Spectroscopy. Aloui, G., Bouabdallah, S., Baltaze, J.P., Herbert Pucheta, J.E., Touil, S., Farjon, J. & Giraud, N.* ChemPhysChem, 20, 1738-1746 (2019) https://dx.doi.org/10.1002/cphc.201900244

A biomimetic strategy for the selective recognition of organophosphates in 100% water: synergies of electrostatic interactions, cavity embedment and metal coordination. Collin, S., Giraud, N., Dumont, E., & Reinaud, O.* Organic Chemistry Frontiers, 6, 1627-1636 (2019) https://dx.doi.org/10.1039/c9qo00263d

2018
Combining pure shift and J‐edited spectroscopies: A strategy for extracting chemical shifts and scalar couplings from highly crowded proton spectra of oligomeric saccharides. Pitoux, D., Hu, Z., Plainchont, B., Merlet, D., Farjon, J., Bonnaffé, D. & Giraud, N.* Magnetic Resonance in Chemistry, 56, 954-962 (2018) https://dx.doi.org/10.1002/mrc.4715

Highly Accurate Quantitative Analysis Of Enantiomeric Mixtures From Spatially Frequency Encoded 1H NMR Spectra. Plainchont, B., Pitoux, D., Cyrille, M. & Giraud, N.* Analytical Chemistry, 90, 1595-1600 (2018) https://dx.doi.org/10.1021/acs.analchem.7b02411

1H NMR analyses of enantiomeric mixtures using chiral liquid crystals (invited paper). Farjon, J. & Giraud, N.* Current Opinion in Colloid and Interface Science, 33, 1-8 (2018) https://dx.doi.org/10.1016/j.cocis.2017.11.001

Before 2017
Paramagnetic DOSY: an Accurate Tool for the Analysis of the Supramolecular Interactions between Lanthanide Complexes and Proteins. Denis-Quanquin, S., Riobé, F., Delsuc, M.A., Maury, O. & Giraud, N.* Chemistry –A European Journal, 22, 18123 – 18131 (2016) https://dx.doi.org/10.1002:chem.201603460

Magnetic Field Dependence of Spatial Frequency Encoding NMR. Plainchont, B., Farjon, J., & Giraud, N.* Encyclopedia of Magnetic Resonance (eMagRes), Vol 5: 1377–1382 (2016) https://dx.doi.org/10.1002/9780470034590.emrstm1528

Correction: Achieving High Resolution And Optimizing Sensitivity In Spatially Frequency Encoding NMR Spectroscopy: From Theory To Practice. Plainchont, B., Pitoux, D., Hamdoun, G., Ouvrard, J.M., Merlet, D., Farjon, J., & Giraud, N.* Physical Chemistry Chemical Physics, 18: 31338 (2016) https://dx.doi.org/10.1039/c6cp90272c

Achieving High Resolution And Optimizing Sensitivity In Spatially Frequency Encoding NMR Spectroscopy: From Theory To Practice. Plainchont, B., Pitoux, D., Hamdoun, G., Ouvrard, J.M., Merlet, D., Farjon, J., & Giraud, N.* Physical Chemistry Chemical Physics, 18: 22827 – 22839 (2016) https://dx.doi.org/10.1039/c6cp01054g

Kinetic commitment in glutamine synthetase catalysis as revealed by 14N/15N and solvent isotope effects Mauve, C., Giraud, N., Boex-Fontvieille E.R.A., Antheaume, I., Tea, I. & Tcherkez, G.* Plant Physiology and Biochemistry, 108: 203-211 (2016) https://dx.doi.org/10.1016/j.plaphy.2016.07.015

Magnetic Field Dependence Of Spatial Frequency Encoding NMR As Probed On An Oligosaccharide. Pitoux, D., Hu, Z., Plainchont, B., Merlet, D., Farjon, J., Bonnaffé, D. & Giraud, N.* Magnetic Resonance in Chemistry, 53: 836-844 (2015) https://dx.doi.org/10.1002/mrc.4281

Pushing The Limits Of Signal Resolution To Make Coupling Measurements Easier. Herbert Pucheta J.E., Pitoux, D., Grison, C.M., Robin, S., Merlet, D., Aitken, D.J., Giraud, N. & Farjon, J.* Chemical Communication, 51: 7939-7942 (2015) https://dx.doi.org/10.1039/c5cc01305d

Fully Resolved NMR Correlation Spectroscopy. Pitoux, D., Plainchont, B., Merlet, D., Hu, Z., Bonnaffé, D. Farjon, J., & Giraud, N.* Chemistry – A European Journal, 21: 9044-9047 (2015) https://dx.doi.org/10.1002/chem.201501182

Simultaneous Determination Of All Forms of Biopterin and Neopterin in Cerebrospinal Fluid. Guibal, P., Leveque, N., Doummar, D., Giraud, N., Roze, E., Rodriguez, D., Couderc, R., Billette De Villemeur, T. & Moussa, F.* ACS Chemical Neuroscience, 5: 533−541 (2014) https://dx.doi.org/10.1021/cn4001928

Exploration of Supramolecular Interactions Involving Tris-Dipicolinate Lanthanide Complexes in Protein Crystals By a Combined Biostructural, Theoretical and NMR Study . Dumont, E., Pompidor, G., D’Aléo, A., Vicat, J., Toupet, L., Maury, O. & Giraud, N.* Physical Chemistry Chemical Physics, 15 (41), 18235-18242 (2013) https://dx.doi.org/10.1039/C3CP53671H

A multidimensional approach to the analysis of NMR titration experiments in the case of a multiple reaction scheme. D’Aléo, A., Dumont, E., Maury, O. & Giraud, N.* Magnetic Resonance in Chemistry, 51 (10):  641–648 (2013) https://dx.doi.org/10.1002/mrc.3994

Combining J-edited and Correlation Spectroscopies Within a Multi-Dimensional Spatial Frequency Encoding: Toward Fully Resolved 1H NMR Spectra. Giraud, N., Pitoux, D., Ouvrard, J.M. & Merlet, D.* Chemistry –A European Journal, 19: 12221-12224 (2013) https://dx.doi.org/10.1002/chem.201302005

NMR in Organic Chemistry. Courtieu, J., Giraud, N., Lafon, O., Lesot, P., Lorthioir, C. & Nuzillard, J.M. Actualité Chimique, 364 : 30-41 (2012) http://www.lactualitechimique.org/La-RMN-en-chimie-organique

Spin-Spin Coupling Edition in Chiral Liquid Crystal NMR Solvent. Merlet, D., Beguin, L., Courtieu, J. & Giraud, N.* Journal of Magnetic Resonance, 209 (2): 315-322 (2011) https://dx.doi.org/10.1016/j.jmr.2011.01.030

Nuclear Magnetic Resonance Using a Spatial Frequency Encoding: Application to J-edited Spectroscopy Along the Sample. Giraud, N., Beguin, L., Courtieu, J. & Merlet, D.* Angewandte Chemie International Edition, 49 (20) :3481-3484 (2010) https://dx.doi.org/10.1002/anie.200907103

Improvements to Selective Refocusing phased (SERFph) experiments. Beguin, L., Giraud, N., Ouvrard, J.M., Courtieu, J. & Merlet, D.* Journal of Magnetic Resonance, 199 (1) : 41-47 (2009) https://dx.doi.org/10.1016/j.jmr.2009.03.012

Application of a 1H -Resolved 2D NMR Experiment to the Visualisation of Enantiomers in a Chiral Environment, using Sample Spatial Encoding and Selective Echoes. Giraud, N., Joos, M., Courtieu, J. & Merlet, D.* Magnetic Resonance in Chemistry, 47 (4) : 300-306 (2009) https://dx.doi.org/10.1002/mrc.2387

Protein Crystallography Through Supramolecular Interactions Between a Lanthanide Complex and Arginin Amino Acid. Pompidor, G., D’Aléo, A., Vicat, J., Toupet, L., Giraud, N.*, Kahn, R.* & Maury, O.* Angewandte Chemie International Edition 47 (18) : 3388-3391 (2008) https://dx.doi.org/10.1002/anie.200704683

The Role of 15N CSA and CSA/Dipole Cross-Correlation in 15N Relaxation in Solid Proteins. Sein, J., Giraud, N., Blackledge, M. & Emsley, L.* Journal of Magnetic Resonance 186 : 26-33 (2007) https://dx.doi.org/10.1016/j.jmr.2007.01.010

Solid-State NMR of a Paramagnetic Protein: Assignment and Study of Human Dimeric Oxidized Cu(II),Zn(II) Superoxide Dismutase (SOD). Pintacuda, G., Giraud, N., Pierattelli, R., Böckmann, A., Bertini, I. & Emsley, L.* Angewandte Chemie International Edition 46 (7) : 1079-1082 (2007) https://dx.doi.org/10.1002/anie.2006.03.093

The Influence of Nitrogen-15 Proton Driven Spin Diffusion on the Measurement of Nitrogen-15 Longitudinal Relaxation Times. Giraud, N., Blackledge, & Emsley, L.* Journal of Magnetic Resonance, 184 (1) : 51-61 (2006) https://dx.doi.org/10.1016/j.jmr.2006.09.015

Observation of Heteronuclear Overhauser Effects Confirms the 15N-1H Dipolar Relaxation Mechanism in a Crystalline Protein. Giraud, N., Sein, J., Pintacuda, G., Lesage, A., Böckmann, A., Blackledge, M. & Emsley, L.* Journal of the American Chemical Society, 128 (38) : 12398-12399 (2006) https://dx.doi.org/10.1021/ja064037g

Quantitative Analysis of Backbone Dynamics in a Crystalline Protein from Nitrogen-15 Spin-Lattice Relaxation. Giraud, N., Blackledge, M., Goldman, M., Böckmann, A., Lesage, A., Penin, F. & Emsley, L. * Journal of the American Chemical Society, 127 (51) : 18190-18201 (2005) https://dx.doi.org/10.1021/ja055182h

Site Specific Backbone Dynamics from a Crystalline Protein by Solid State NMR Spectroscopy. Giraud, N., Böckmann, A., Lesage, A., Penin, F., Blackledge, M. & Emsley, L.* Journal of the American Chemical Society126 (37) : 11422-11423 (2004). https://dx.doi.org/10.1021/ja046578g

Transverse Dephasing Optimized Solid State NMR Spectroscopy. De Paëpe, G. , Giraud, N., Lesage, A., Hodgkinson, P., Böckmann, A. & Emsley, L.* Journal of the American Chemical Society 125 (46) : 13938-13939 (2003). https://dx.doi.org/10.1021/ja037213j

Solid-state NMR sequential resonance assignments and conformational analysis of the 2×10.4 kDa dimeric form of the Bacillus Subtilis protein, Crh. Böckmann, A.*, Lange, A., Galinier, A., Luca, S., Giraud, N., Juy, M., Heise, H., Montserret, R., Penin, F., & Baldus, M. Journal of Biomolecular NMR 27 (4) : 323-339 (2003). https://dx.doi.org/10.1023/A:1025820611009