Université de Paris – LCBPT UMR 8601
45 rue des Saints Pères, 75006 Paris

Phone: +33 1 76 53 42 14 • Room R171

Biography

Nicolas Giraud graduated in chemistry from the Ecole Normale Supérieure (Lyon). His early interest for nuclear magnetic resonance stemmed from his internship carried out in 2000 in the group of Prof. Horst Kessler at Technical University of Munich (Germany), on the structural analysis of synthetic peptides. He completed a PhD on Biological NMR at Université Claude Bernard (Lyon) in 2005, in the groups of Prof. Lyndon Emsley, Dr. Martin Blackledge, and Dr. François Penin. In 2006, he moved to the Université Paris Sud (now Université Paris Saclay) where he was appointed Associate Professor in the group led by Prof. Jacques Courtieu. He has worked on the development of high-resolution NMR techniques mainly in the field of chiral analysis and supramolecular interactions. 

    He moved to Université de Paris (Paris Descartes) and became Professor of Chemistry in 2017. He is head of the research group NMR of Biological Substances, and coordinator of the research team Bio-Spectroscopy (together with Prof. Claire Mangeney). His research interests include the study of challenging molecular or supramolecular systems in the field of Health Sciences, with a particular emphasis on the development of analytical methods targeting their dynamic and structural features. He is teaching chemistry in the Department of Fundamental and Biomedical Sciences (Faculty of Sciences). He is co-chairing the first year of the Master Program “Frontiers in Chemistry” (together with Dr. Aurélie Perrier).  

    Over the last years, he has been coordinator or PI in several granted projects, both for research and educational purposes. He has contributed to organizing several national and international conferences in the field of magnetic resonance, as well as thematic schools. He was member of the organizing committee of the Alpine Conference on Solid-State NMR (now Alpine Conference on Magnetic Resonance in Solids) from 2007 to 2017. He became member of the executive board of the GERM (French Group for the Study of Magnetic Resonance) in 2014, and has been its elected president from 2016 to 2018.

 

Curriculum Vitae

 Keywords 

Analytical chemistry • methodological development • ultrahigh-resolution NMR • paramagnetic NMR • nuclear spin relaxation • exchange spectroscopy • diffusion ordered NMR • biomolecules • complex mixtures • metabolites • proteins • supramolecular interactions • dynamic and structural analysis • quantitative analysis 

 

Teaching Activities 

  • Lectures, tutorials and practical works in nuclear magnetic resonance (Master Frontiers in Chemistry)
  • Lectures and tutorials in electrochemistry (Licence in Chemistry)

Publications

2021

Ultrahigh-Resolution NMR with Water Signal Suppression for a Deeper Understanding of the Action of Antimetabolic Drugs on Dif-fuse Large B-Cell Lymphoma
Bertho, G., Lordello, L., Chen, X., Lucas-Torres, C., Oumezziane, I.E., Caradeuc, C., Baudin, M., Nuan-Aliman, S., Thieblemont, C., Baud, V.* and Giraud, N.*
Journal of Proteome Research, in Press (2021) DOI: https://doi.org/10.1021/acs.jproteome.1c00914

Structural Analysis of Unstable Norbixin Isomers Guided by Pure Shift Nuclear Magnetic Resonance.
Bertho, G.* Oumezziane, I.E., Caradeuc, C., Guibout, L., Balducci, C., Dinan, L., Dilda, P.J., Camelo, S., Lafont, R., and Giraud, N.*
Magnetic Resonance in Chemistry, Advance Article (2021) DOI: https://doi.org/10.1002/mrc.5252

Capturing The Dynamic Association Between A Tris-Dipicolinate Lanthanide Complex And A Decapeptide: A Combined Paramagnetic NMR And Molecular Dynamics Exploration.
Denis-Quanquin, S., Bartocci, A., Szczepaniak, F., Riobé, F., Maury, O., Dumont, E.*, Giraud, N.*
Physical Chemistry Chemical Physics, 23, 11224-11232 (2021)
https://doi.org/10.1039/D0CP06570F

Solid-State versus Solution Investigation of a Luminescent Chiral BINOL-Derived Bisphosphate Single-Molecule Magnet
Mattei C.A., Montigaud, V., Gendron F., Denis-Quanquin S., Dorcet V., Giraud N., Riobé F., Argouarch G., Maury O., Le Guennic B.,* Cador O., Lalli C., Pointillart F.*
Inorg. Chem. Front., 8, 947-962 (2021)
https://doi.org/10.1039/D0QI01192D

2020

Mn(I) Complex Redox Potential Tunability by Remote Lewis Acid Interaction.
Srinivasan, A. Campos, J., Giraud, N., Robert M. and Rivada-Wheelaghan O.* Dalton Trans., 2020,49, 16623-16626 (2020https://dx.doi.org/10.1039/D0DT02467H

Multinuclear NMR in Polypeptide Liquid Crystals: Three Fertile Decades of Methodological Developments and Analytical Challenges. Lesot, P.*, Aroulanda, C., Berdagué, P., Meddour, A., J., Merlet, D., Farjon, J., Giraud, N., & Lafon, O. Progress in Nuclear Magnetic Resonance Spectroscopy, 116, 85-154 (2020) https://dx.doi.org/10.1016/j.pnmrs.2019.10.001

2019

Monitoring Conformational Changes in an Enzyme Conversion Inhibitor Using Pure Shift Exchange NMR Spectroscopy. Aloui, G., Bouabdallah, S., Baltaze, J.P., Herbert Pucheta, J.E., Touil, S., Farjon, J. & Giraud, N.* ChemPhysChem, 20, 1738-1746 (2019) https://dx.doi.org/10.1002/cphc.201900244

A biomimetic strategy for the selective recognition of organophosphates in 100% water: synergies of electrostatic interactions, cavity embedment and metal coordination. Collin, S., Giraud, N., Dumont, E., & Reinaud, O.* Organic Chemistry Frontiers, 6, 1627-1636 (2019) https://dx.doi.org/10.1039/c9qo00263d

2018
Combining pure shift and J‐edited spectroscopies: A strategy for extracting chemical shifts and scalar couplings from highly crowded proton spectra of oligomeric saccharides. Pitoux, D., Hu, Z., Plainchont, B., Merlet, D., Farjon, J., Bonnaffé, D. & Giraud, N.* Magnetic Resonance in Chemistry, 56, 954-962 (2018) https://dx.doi.org/10.1002/mrc.4715

Highly Accurate Quantitative Analysis Of Enantiomeric Mixtures From Spatially Frequency Encoded 1H NMR Spectra. Plainchont, B., Pitoux, D., Cyrille, M. & Giraud, N.* Analytical Chemistry, 90, 1595-1600 (2018) https://dx.doi.org/10.1021/acs.analchem.7b02411

1H NMR analyses of enantiomeric mixtures using chiral liquid crystals (invited paper). Farjon, J. & Giraud, N.* Current Opinion in Colloid and Interface Science, 33, 1-8 (2018) https://dx.doi.org/10.1016/j.cocis.2017.11.001

Before 2017
Paramagnetic DOSY: an Accurate Tool for the Analysis of the Supramolecular Interactions between Lanthanide Complexes and Proteins. Denis-Quanquin, S., Riobé, F., Delsuc, M.A., Maury, O. & Giraud, N.* Chemistry –A European Journal, 22, 18123 – 18131 (2016) https://dx.doi.org/10.1002:chem.201603460

Magnetic Field Dependence of Spatial Frequency Encoding NMR. Plainchont, B., Farjon, J., & Giraud, N.* Encyclopedia of Magnetic Resonance (eMagRes), Vol 5: 1377–1382 (2016) https://dx.doi.org/10.1002/9780470034590.emrstm1528

Correction: Achieving High Resolution And Optimizing Sensitivity In Spatially Frequency Encoding NMR Spectroscopy: From Theory To Practice. Plainchont, B., Pitoux, D., Hamdoun, G., Ouvrard, J.M., Merlet, D., Farjon, J., & Giraud, N.* Physical Chemistry Chemical Physics, 18: 31338 (2016) https://dx.doi.org/10.1039/c6cp90272c

Achieving High Resolution And Optimizing Sensitivity In Spatially Frequency Encoding NMR Spectroscopy: From Theory To Practice. Plainchont, B., Pitoux, D., Hamdoun, G., Ouvrard, J.M., Merlet, D., Farjon, J., & Giraud, N.* Physical Chemistry Chemical Physics, 18: 22827 – 22839 (2016) https://dx.doi.org/10.1039/c6cp01054g

Kinetic commitment in glutamine synthetase catalysis as revealed by 14N/15N and solvent isotope effects Mauve, C., Giraud, N., Boex-Fontvieille E.R.A., Antheaume, I., Tea, I. & Tcherkez, G.* Plant Physiology and Biochemistry, 108: 203-211 (2016) https://dx.doi.org/10.1016/j.plaphy.2016.07.015

Magnetic Field Dependence Of Spatial Frequency Encoding NMR As Probed On An Oligosaccharide. Pitoux, D., Hu, Z., Plainchont, B., Merlet, D., Farjon, J., Bonnaffé, D. & Giraud, N.* Magnetic Resonance in Chemistry, 53: 836-844 (2015) https://dx.doi.org/10.1002/mrc.4281

Pushing The Limits Of Signal Resolution To Make Coupling Measurements Easier. Herbert Pucheta J.E., Pitoux, D., Grison, C.M., Robin, S., Merlet, D., Aitken, D.J., Giraud, N. & Farjon, J.* Chemical Communication, 51: 7939-7942 (2015) https://dx.doi.org/10.1039/c5cc01305d

Fully Resolved NMR Correlation Spectroscopy. Pitoux, D., Plainchont, B., Merlet, D., Hu, Z., Bonnaffé, D. Farjon, J., & Giraud, N.* Chemistry – A European Journal, 21: 9044-9047 (2015) https://dx.doi.org/10.1002/chem.201501182

Simultaneous Determination Of All Forms of Biopterin and Neopterin in Cerebrospinal Fluid. Guibal, P., Leveque, N., Doummar, D., Giraud, N., Roze, E., Rodriguez, D., Couderc, R., Billette De Villemeur, T. & Moussa, F.* ACS Chemical Neuroscience, 5: 533−541 (2014) https://dx.doi.org/10.1021/cn4001928

Exploration of Supramolecular Interactions Involving Tris-Dipicolinate Lanthanide Complexes in Protein Crystals By a Combined Biostructural, Theoretical and NMR Study . Dumont, E., Pompidor, G., D’Aléo, A., Vicat, J., Toupet, L., Maury, O. & Giraud, N.* Physical Chemistry Chemical Physics, 15 (41), 18235-18242 (2013) https://dx.doi.org/10.1039/C3CP53671H

A multidimensional approach to the analysis of NMR titration experiments in the case of a multiple reaction scheme. D’Aléo, A., Dumont, E., Maury, O. & Giraud, N.* Magnetic Resonance in Chemistry, 51 (10):  641–648 (2013) https://dx.doi.org/10.1002/mrc.3994

Combining J-edited and Correlation Spectroscopies Within a Multi-Dimensional Spatial Frequency Encoding: Toward Fully Resolved 1H NMR Spectra. Giraud, N., Pitoux, D., Ouvrard, J.M. & Merlet, D.* Chemistry –A European Journal, 19: 12221-12224 (2013) https://dx.doi.org/10.1002/chem.201302005

NMR in Organic Chemistry. Courtieu, J., Giraud, N., Lafon, O., Lesot, P., Lorthioir, C. & Nuzillard, J.M. Actualité Chimique, 364 : 30-41 (2012) http://www.lactualitechimique.org/La-RMN-en-chimie-organique

Spin-Spin Coupling Edition in Chiral Liquid Crystal NMR Solvent. Merlet, D., Beguin, L., Courtieu, J. & Giraud, N.* Journal of Magnetic Resonance, 209 (2): 315-322 (2011) https://dx.doi.org/10.1016/j.jmr.2011.01.030

Nuclear Magnetic Resonance Using a Spatial Frequency Encoding: Application to J-edited Spectroscopy Along the Sample. Giraud, N., Beguin, L., Courtieu, J. & Merlet, D.* Angewandte Chemie International Edition, 49 (20) :3481-3484 (2010) https://dx.doi.org/10.1002/anie.200907103

Improvements to Selective Refocusing phased (SERFph) experiments. Beguin, L., Giraud, N., Ouvrard, J.M., Courtieu, J. & Merlet, D.* Journal of Magnetic Resonance, 199 (1) : 41-47 (2009) https://dx.doi.org/10.1016/j.jmr.2009.03.012

Application of a 1H -Resolved 2D NMR Experiment to the Visualisation of Enantiomers in a Chiral Environment, using Sample Spatial Encoding and Selective Echoes. Giraud, N., Joos, M., Courtieu, J. & Merlet, D.* Magnetic Resonance in Chemistry, 47 (4) : 300-306 (2009) https://dx.doi.org/10.1002/mrc.2387

Protein Crystallography Through Supramolecular Interactions Between a Lanthanide Complex and Arginin Amino Acid. Pompidor, G., D’Aléo, A., Vicat, J., Toupet, L., Giraud, N.*, Kahn, R.* & Maury, O.* Angewandte Chemie International Edition 47 (18) : 3388-3391 (2008) https://dx.doi.org/10.1002/anie.200704683

The Role of 15N CSA and CSA/Dipole Cross-Correlation in 15N Relaxation in Solid Proteins. Sein, J., Giraud, N., Blackledge, M. & Emsley, L.* Journal of Magnetic Resonance 186 : 26-33 (2007) https://dx.doi.org/10.1016/j.jmr.2007.01.010

Solid-State NMR of a Paramagnetic Protein: Assignment and Study of Human Dimeric Oxidized Cu(II),Zn(II) Superoxide Dismutase (SOD). Pintacuda, G., Giraud, N., Pierattelli, R., Böckmann, A., Bertini, I. & Emsley, L.* Angewandte Chemie International Edition 46 (7) : 1079-1082 (2007) https://dx.doi.org/10.1002/anie.2006.03.093

The Influence of Nitrogen-15 Proton Driven Spin Diffusion on the Measurement of Nitrogen-15 Longitudinal Relaxation Times. Giraud, N., Blackledge, & Emsley, L.* Journal of Magnetic Resonance, 184 (1) : 51-61 (2006) https://dx.doi.org/10.1016/j.jmr.2006.09.015

Observation of Heteronuclear Overhauser Effects Confirms the 15N-1H Dipolar Relaxation Mechanism in a Crystalline Protein. Giraud, N., Sein, J., Pintacuda, G., Lesage, A., Böckmann, A., Blackledge, M. & Emsley, L.* Journal of the American Chemical Society, 128 (38) : 12398-12399 (2006) https://dx.doi.org/10.1021/ja064037g

Quantitative Analysis of Backbone Dynamics in a Crystalline Protein from Nitrogen-15 Spin-Lattice Relaxation. Giraud, N., Blackledge, M., Goldman, M., Böckmann, A., Lesage, A., Penin, F. & Emsley, L. * Journal of the American Chemical Society, 127 (51) : 18190-18201 (2005) https://dx.doi.org/10.1021/ja055182h

Site Specific Backbone Dynamics from a Crystalline Protein by Solid State NMR Spectroscopy. Giraud, N., Böckmann, A., Lesage, A., Penin, F., Blackledge, M. & Emsley, L.* Journal of the American Chemical Society126 (37) : 11422-11423 (2004). https://dx.doi.org/10.1021/ja046578g

Transverse Dephasing Optimized Solid State NMR Spectroscopy. De Paëpe, G. , Giraud, N., Lesage, A., Hodgkinson, P., Böckmann, A. & Emsley, L.* Journal of the American Chemical Society 125 (46) : 13938-13939 (2003). https://dx.doi.org/10.1021/ja037213j

Solid-state NMR sequential resonance assignments and conformational analysis of the 2×10.4 kDa dimeric form of the Bacillus Subtilis protein, Crh. Böckmann, A.*, Lange, A., Galinier, A., Luca, S., Giraud, N., Juy, M., Heise, H., Montserret, R., Penin, F., & Baldus, M. Journal of Biomolecular NMR 27 (4) : 323-339 (2003). https://dx.doi.org/10.1023/A:1025820611009