Bio-Spectroscopy

biospectroscopy Recent developments in technology, experimental methodologies, but also data processing management and analysis have opened the way to new breakthroughs in the field of biospectroscopy. However, many challenges remain in biomarker identification, sample preparation, instrumentation, biomarkers identification and data handling. With our broad range of state-of-the-art equipment (NMR, EPR and micro-Raman) and robust collaborations with doctors and biologists, the BIOSPECTROSCOPY team drives the development of advanced biospectroscopy analysis and imaging. Moreover, we develop a synergetic approach by combining different types of spectroscopiesy on important health issues. To meet these challenges, the team is organized into three thematic groups, as described below.

Nuclear Magnetic Resonance Spectroscopy

Research projects in the NMR group target structural and dynamic analyses of challenging molecular assemblies in the fields of chemistry, biology and medicine, ranging from the study of biomolecular interactions to metabolomics studies, and the development of new techniques to acquire ultra high-resolution spectra. We are also working on the development of methods for boosting the intrinsic sensitivity of NMR analyses, based on Dissolution Dynamic Nuclear Polarization (D-DNP).

EPR and Imaging

The EPR group develops analytical methods based on Electron Paramagnetic Resonance spectroscopy and imaging to study very diverse samples of interest (ranging from materials  to humans). Their research activity covers a wide range of projects ranging from mathematics to chemistry, with a particular emphasis on the development of clinical EPR. 

 Nano Bio-Spectroscopy

The interface of nano-bioscience and medicine is a rapidly evolving research field which offers unprecedented opportunities in personalized medicine. However, it requires substantial efforts (i) to improve our understanding of interactions between nanomaterials and biological systems and (ii) to be able to modulate the nano-bio interface. The work of the Nano Bio-Spectroscopy group focuses on the design of innovative nanomaterials and the study of their interactions with different biological targets (cells, tissues…) aiming at developing a new generation of probes and sensors for molecular imaging, diagnostics and drug delivery.

Main Publications

I. Tijunelyte, I. Kherbouche et al. Multi-functionalization of lithographically designed gold nanodisks by plasmon-mediated reduction of aryl diazonium salts . Nanoscale Horiz., 2018, 3, 53-57.
R. Rebois, D. Onidas, C. Marcott et al. Chloroform induces outstanding crystallization of poly(hydroxybutyrate) (PHB) vesicles within bacteria. Anal. Bioanal., Chem. 2017, 409(9), 2353-2361.
M. Nguyen, A. Lamouri, C. Salameh, G. Levi et al. Plasmon-Mediated Chemical Surface Functionalization at the Nanoscale, Nanoscale, 2016, 8, 8633-8640.
M. Nguyen, X. Sun, E. Lacaze, P. Winkler et al. Engineering Thermo-switchable Lithographic Hybrid Gold Nanorods for Sensing and Active Plasmonics Applications. ACS Photonics, 2015, 2 (8), 1199–1208.
R. Ahmad, N. Griffete, A. Lamouri, N. Félidj et al. Nanocomposites of gold nanoparticles@molecularly imprinted polymers: chemistry, processing and applications in sensors. Chem. Mater, 2015, 27 (16), 5464–5478

Bio-Spectroscopy

TEAM COMPOSITION

Research

Recent developments in technology, experimental methodologies, but also data processing management and analysis have opened the way to new breakthroughs in the field of biospectroscopy. biospectroscopyHowever, many challenges remain in biomarker identification, sample preparation, instrumentation, biomarkers identification and data handling. With our broad range of state-of-the-art equipment (NMR, EPR and micro-Raman) and robust collaborations with doctors and biologists, the BIOSPECTROSCOPY team drives the development of advanced biospectroscopy analysis and imaging. Moreover, we develop a synergetic approach by combining different types of spectroscopiesy on important health issues. To meet these challenges, the team is organized into three thematic groups, as described below.

Nuclear Magnetic Resonance Spectroscopy

Research projects in the NMR group target structural and dynamic analyses of challenging molecular assemblies in the fields of chemistry, biology and medicine, ranging from the study of biomolecular interactions to metabolomics studies, and the development of new techniques to acquire ultra high-resolution spectra. We are also working on the development of methods for boosting the intrinsic sensitivity of NMR analyses, based on Dissolution Dynamic Nuclear Polarization (D-DNP).

EPR and Imaging

The EPR group develops analytical methods based on Electron Paramagnetic Resonance spectroscopy and imaging to study very diverse samples of interest (ranging from materials  to humans). Their research activity covers a wide range of projects ranging from mathematics to chemistry, with a particular emphasis on the development of clinical EPR. 

 Nano Bio-Spectroscopy

The interface of nano-bioscience and medicine is a rapidly evolving research field which offers unprecedented opportunities in personalized medicine. However, it requires substantial efforts (i) to improve our understanding of interactions between nanomaterials and biological systems and (ii) to be able to modulate the nano-bio interface. The work of the Nano Bio-Spectroscopy group focuses on the design of innovative nanomaterials and the study of their interactions with different biological targets (cells, tissues…) aiming at developing a new generation of probes and sensors for molecular imaging, diagnostics and drug delivery.

Main Publications

I. Tijunelyte, I. Kherbouche et al. Multi-functionalization of lithographically designed gold nanodisks by plasmon-mediated reduction of aryl diazonium salts . Nanoscale Horiz., 2018, 3, 53-57.
R. Rebois, D. Onidas, C. Marcott et al. Chloroform induces outstanding crystallization of poly(hydroxybutyrate) (PHB) vesicles within bacteria. Anal. Bioanal., Chem. 2017, 409(9), 2353-2361.
M. Nguyen, A. Lamouri, C. Salameh, G. Levi et al. Plasmon-Mediated Chemical Surface Functionalization at the Nanoscale, Nanoscale, 2016, 8, 8633-8640.
M. Nguyen, X. Sun, E. Lacaze, P. Winkler et al. Engineering Thermo-switchable Lithographic Hybrid Gold Nanorods for Sensing and Active Plasmonics Applications. ACS Photonics, 2015, 2 (8), 1199–1208.
R. Ahmad, N. Griffete, A. Lamouri, N. Félidj et al. Nanocomposites of gold nanoparticles@molecularly imprinted polymers: chemistry, processing and applications in sensors. Chem. Mater, 2015, 27 (16), 5464–5478