Nicolas GIRAUD
Professor
Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques
UMR CNRS 8601
Université Paris Cité
45 Rue des Saints Pères
75270 Paris Cedex 06
Biography
Nicolas Giraud graduated in chemistry from the Ecole Normale Supérieure de Lyon. His early interest for nuclear magnetic resonance stemmed from his internship carried out in 2000 in the group of Prof. Horst Kessler at Technical University of Munich (Germany), on the structural analysis of synthetic peptides. He completed a PhD on Biological NMR at Université Claude Bernard (Lyon) in 2005, in the groups of Prof. Lyndon Emsley, Dr. Martin Blackledge, and Dr. François Penin. In 2006, he moved to the Université Paris Sud (now Université Paris Saclay) where he was appointed Associate Professor in the group led by Prof. Jacques Courtieu. He has worked on the development of high-resolution NMR techniques mainly in the field of chiral analysis and supramolecular interactions.
He moved to Université Paris Cité (Paris Descartes) where he became Professor of Chemistry in 2017. He is head of the research group NMR of Biological Substances, and coordinator of the research team Bio-Spectroscopy (together with Prof. Claire Mangeney). His research interests include the study of challenging molecular or supramolecular systems in the field of Health Sciences, with a particular emphasis on the development of analytical methods targeting their dynamic and structural features. He is teaching chemistry in the Department of Fundamental and Biomedical Sciences (Faculty of Sciences). He is co-chairing the Master Program “Frontiers in Chemistry” (together with Prof. Aurélie Perrier).
Over the last years, he has been coordinator or PI in several granted projects, both for research and educational purposes. He has contributed to organizing national and international conferences in the field of magnetic resonance, as well as thematic schools. He was member of the organizing committee of the Alpine Conference on Solid-State NMR (now Alpine Conference on Magnetic Resonance in Solids) from 2007 to 2017. He became member of the executive board of the GERM (French Group for the Study of Magnetic Resonance) in 2014, and has been its elected president from 2016 to 2018.
Curriculum Vitae
Since 2017 Professor of Chemistry (section CNU 31) – Université Paris Cité
Elected member, Academic Senate
Elected member, Council of the Faculty of Sciences
Head, research group on NMR of Biological Substances
Coordinator, team Bio-Spectroscopies, UMR CNRS 8601
Program co-chair, Master Frontiers in Chemistry
2006 – 2017 Associate Professor (HDR) – Université Paris-Saclay
2005 – 2006 Agrégé Préparateur – Ecole Normale Supérieure de Lyon
2002 – 2005 PhD Student – University Claude Bernard Lyon 1
1998 – 2002 Elève Normalien – Ecole Normale Supérieure de Lyon
Teaching Activities
Nuclear Magnetic Resonance: Lectures, tutorials and practical works (Master Frontiers in Chemistry)
Chemistry: Lectures and tutorials (Licence in Chemistry)
Research Themes
Analytical chemistry • Methodological development • Ultrahigh-resolution NMR • Paramagnetic NMR • Nuclear spin relaxation • Biomolecules • Complex mixtures • Metabolites • Supramolecular interactions • Dynamic and structural analysis • Quantitative analysis
Publications
2024
Simultaneous observation of the anomerization and reaction rates of enzymatic dehydrogenation of Glucose-6-Phosphate by dissolution DNP
M. Soussi-Therond, A. Razanahoera, Y. Zhang, M. Baudin, E. Miclet, N. Giraud, D. Abergel*
Journal of the American Chemical Society 2024, in press. DOI : 10.1021/jacs.4c12904
Les complexes de lanthanide, des sondes polyvalentes de la structure des protéines
E. Dumont, O. Maury N. Giraud*
Actualité Chimique, 499, 54, 2024, https://new.societechimiquedefrance.fr/numero/les-methodes-pour-la-chimie-bioinorganique-p54-n499/
Revisiting the nature and pharmacodynamics of tacrolimus metabolites
R. Mevizoud, H. Aouad, F.L. Sauvage, H. Arnion, E. Pinault, J.S. Bernard, G. Bertho, N. Giraud, R. Alves de Sousa, A. Lopez-Noriega, F. Di Meo, M. Camapana, P. Marquet
Pharmacological Research 2024, 209, 107438. DOI : 10.1016/j.phrs.2024.107438
Phytochemical Study of the Anthelminthic Potential of Guadeloupean Plant Biodiversity
T. Cabald, C. Marie-Magdeleine, L. Philibert, C. Caradeuc, G. Bertho, N. Giraud, G. Cebrián-Torrejón, M. Sylvestre
Pharmaceuticals 2024, 17, 774. DOI : 10.3390/ph17060774
The analysis of the skeletal muscle metabolism is crucial for designing optimal exercise paradigms in type 2 diabetes mellitus
E. Abi Akar, L. Weill, M. El Khoury, C. Caradeuc, G. Bertho, S. Boutary, C. Bezier, Z. Clerc, D. Sapaly, S. Bendris, F. Cheguillaume, N. Giraud, A. A. Eid, F. Charbonnier, O. Biondi
Molecular Medicine 30, 80 (2024) DOI: 10.1186/s10020-024-00850-7
Glutamine: A key player in human metabolism as revealed by hyperpolarized magnetic resonance
Dos Santos, K., Bertho, G., Baudin, M., Giraud, N.*
Progress in Nuclear Magnetic Resonance Spectroscopy, 144-145, 15-39 (2024) DOI : 10.1016/j.pnmrs.2024.05.003
One touch is all it takes: the supramolecular interaction between ubiquitin and lanthanide complexes revisited by paramagnetic NMR and molecular dynamics
K. Dos Santos, A. Bartocci, N. Gillet, S. Denis-Quanquin, A. Roux, E. Lin, Z. Xu, R. Finizola,P. Chedozeau,X. Chen, C. Caradeuc, M. Baudin, G. Bertho, F. Riobé, O. Maury, E. Dumont* and N. Giraud* ,
Physical Chemistry Chemical Physics, 26, 14573-14581 (2024) DOI: 10.1039/D4CP00463A
Selected as a 2024 PCCP HOT ARTICLE
Lipoproteome dysregulation precedes conversion to psychosis in ultra-high-risk subjects,
M.T. Avella, G. Bertho, O. Kébir, C. Caradeuc, M.O. Krebs, N. Giraud, B. Chaumette, A. Frajerman,
Neuroscience Applied, Volume 3, Supplement 1, (2024),103948 DOI: 10.1016/j.nsa.2024.103948
2023
Guest exchange in a biomimetic ZnII cavity-complex: kinetic control by a catalytic water, through pore selection, 2nd sphere assistance, and induced-fit processes
Nyssen, N., Giraud, N., Wouters, J., Jabin, I., Leherte, L. & Reinaud, O.*
Inorganic Chemistry Frontiers, in Press (2023) DOI : 10.1039/D3QI01271A
NMR metabolomics study of the chronic low-dose exposure to a cocktail of Persistent Organic Pollutants
Lucas-Torres, C., Caradeuc, C., Prieur, L., Djemai, H., Layale, Y., Noirez, P., Coumoul, X., Audouze, K., Giraud, N., & Bertho, G.*
NMR in Biomedicine, in Press (2023) DOI : 10.1002/nbm.5006
Present and Future of Pure Shift NMR in Metabolomics
Chen, X., Bertho, G., Caradeuc, C., Giraud, N.* and Lucas-Torres, C.*,
Magnetic Resonance in Chemistry Metabolomics Special Issue: Frontiers in NMR Metabolomics, invited review (2023) DOI: 10.1002/mrc.5356
A Toolbox For Glutamine Use In Dissolution Dynamic Nuclear Polarization: From Enzymatic Reaction Monitoring To The Study Of Cellular Metabolic Pathways And Imaging.
Dos Santos, K, Bertho, G., Caradeuc, C., Baud, V., Montagne, A., Abergel, D., Giraud, N.* & Baudin, M.*
ChemPhysChem (2023) DOI: 10.1002/cphc.202300151
2022
Absolute Metabolite Quantification Using Pure Shift NMR: Towards Quantitative Metabolic Profiling of Aqueous Biological Samples
Chen, X., Caradeuc, C., Montagne, A., Baud, V., Bertho, G., Lucas-Torres, C.*, Giraud, N.*
Anal. Chem. 94, 43, 14974–14984 (2022) DOI: 10.1021/acs.analchem.2c02823
Synthesis and Characterization of Novel Conformers of (E)-2-(3-nitro-H-imidazo[1,2-a]pyridin-2-ylthio)-N’-benzylideneacetohydrazide Derivatives
Evrard, A., Coulibaly, S.*, Coulibali, S *, Kouassi, S., Achi, P.A., Giraud, N. and Bertho, G.
Magnetic Resonance in Chemistry, 60, 1157-1170 (2022) DOI: 10.1002/mrc.5308
Lanthanide tris-dipicolinate: a do-it-all complex?
Denis Quanquin, S., D’Aléo, A., Balogh, C., N’Dala Louika, I., Salaam, J., le Guennic, B., Thieuleux, C., Camp, C., Veyre, L., Costuas, K., Rigaut, S., Le Bozec, H., Andraud, C., Dumont, E., Gilet, N., Chapelle, C., Girard, E., Giraud, N., Pilet, G., Riobé, F., Maury, O*
Actualité Chimique, 475, 12 (2022)
Ultrahigh-Resolution NMR with Water Signal Suppression for a Deeper Understanding of the Action of Antimetabolic Drugs on Dif-fuse Large B-Cell Lymphoma
Bertho, G., Lordello, L., Chen, X., Lucas-Torres, C., Oumezziane, I.E., Caradeuc, C., Baudin, M., Nuan-Aliman, S., Thieblemont, C., Baud, V.* and Giraud, N.*
Journal of Proteome Research, 21, 4, 1041-1051 (2022) DOI: https://doi.org/10.1021/acs.jproteome.1c00914
Structural Analysis of Unstable Norbixin Isomers Guided by Pure Shift Nuclear Magnetic Resonance.
Bertho, G.* Oumezziane, I.E., Caradeuc, C., Guibout, L., Balducci, C., Dinan, L., Dilda, P.J., Camelo, S., Lafont, R., and Giraud, N.*
Magnetic Resonance in Chemistry, 60, 504-514 (2022) DOI: https://doi.org/10.1002/mrc.5252
2021
Capturing The Dynamic Association Between A Tris-Dipicolinate Lanthanide Complex And A Decapeptide: A Combined Paramagnetic NMR And Molecular Dynamics Exploration.
Denis-Quanquin, S., Bartocci, A., Szczepaniak, F., Riobé, F., Maury, O., Dumont, E.*, Giraud, N.*
Physical Chemistry Chemical Physics, 23, 11224-11232 (2021)
https://doi.org/10.1039/D0CP06570F
Solid-State versus Solution Investigation of a Luminescent Chiral BINOL-Derived Bisphosphate Single-Molecule Magnet
Mattei C.A., Montigaud, V., Gendron F., Denis-Quanquin S., Dorcet V., Giraud N., Riobé F., Argouarch G., Maury O., Le Guennic B.,* Cador O., Lalli C., Pointillart F.*
Inorg. Chem. Front., 8, 947-962 (2021)
https://doi.org/10.1039/D0QI01192D
2020
Mn(I) Complex Redox Potential Tunability by Remote Lewis Acid Interaction.
Srinivasan, A. Campos, J., Giraud, N., Robert M. and Rivada-Wheelaghan O.* Dalton Trans., 2020,49, 16623-16626 (2020) https://dx.doi.org/10.1039/D0DT02467H
Multinuclear NMR in Polypeptide Liquid Crystals: Three Fertile Decades of Methodological Developments and Analytical Challenges. Lesot, P.*, Aroulanda, C., Berdagué, P., Meddour, A., J., Merlet, D., Farjon, J., Giraud, N., & Lafon, O. Progress in Nuclear Magnetic Resonance Spectroscopy, 116, 85-154 (2020) https://dx.doi.org/10.1016/j.pnmrs.2019.10.001
2019
Monitoring Conformational Changes in an Enzyme Conversion Inhibitor Using Pure Shift Exchange NMR Spectroscopy. Aloui, G., Bouabdallah, S., Baltaze, J.P., Herbert Pucheta, J.E., Touil, S., Farjon, J. & Giraud, N.* ChemPhysChem, 20, 1738-1746 (2019) https://dx.doi.org/10.1002/cphc.201900244
A biomimetic strategy for the selective recognition of organophosphates in 100% water: synergies of electrostatic interactions, cavity embedment and metal coordination. Collin, S., Giraud, N., Dumont, E., & Reinaud, O.* Organic Chemistry Frontiers, 6, 1627-1636 (2019) https://dx.doi.org/10.1039/c9qo00263d
2018
Highly Accurate Quantitative Analysis Of Enantiomeric Mixtures From Spatially Frequency Encoded 1H NMR Spectra. Plainchont, B., Pitoux, D., Cyrille, M. & Giraud, N.* Analytical Chemistry, 90, 1595-1600 (2018) https://dx.doi.org/10.1021/acs.analchem.7b02411
1H NMR analyses of enantiomeric mixtures using chiral liquid crystals (invited paper). Farjon, J. & Giraud, N.* Current Opinion in Colloid and Interface Science, 33, 1-8 (2018) https://dx.doi.org/10.1016/j.cocis.2017.11.001
Before 2017
Magnetic Field Dependence of Spatial Frequency Encoding NMR. Plainchont, B., Farjon, J., & Giraud, N.* Encyclopedia of Magnetic Resonance (eMagRes), Vol 5: 1377–1382 (2016) https://dx.doi.org/10.1002/9780470034590.emrstm1528
Correction: Achieving High Resolution And Optimizing Sensitivity In Spatially Frequency Encoding NMR Spectroscopy: From Theory To Practice. Plainchont, B., Pitoux, D., Hamdoun, G., Ouvrard, J.M., Merlet, D., Farjon, J., & Giraud, N.* Physical Chemistry Chemical Physics, 18: 31338 (2016) https://dx.doi.org/10.1039/c6cp90272c
Achieving High Resolution And Optimizing Sensitivity In Spatially Frequency Encoding NMR Spectroscopy: From Theory To Practice. Plainchont, B., Pitoux, D., Hamdoun, G., Ouvrard, J.M., Merlet, D., Farjon, J., & Giraud, N.* Physical Chemistry Chemical Physics, 18: 22827 – 22839 (2016) https://dx.doi.org/10.1039/c6cp01054g
Kinetic commitment in glutamine synthetase catalysis as revealed by 14N/15N and solvent isotope effects Mauve, C., Giraud, N., Boex-Fontvieille E.R.A., Antheaume, I., Tea, I. & Tcherkez, G.* Plant Physiology and Biochemistry, 108: 203-211 (2016) https://dx.doi.org/10.1016/j.plaphy.2016.07.015
Magnetic Field Dependence Of Spatial Frequency Encoding NMR As Probed On An Oligosaccharide. Pitoux, D., Hu, Z., Plainchont, B., Merlet, D., Farjon, J., Bonnaffé, D. & Giraud, N.* Magnetic Resonance in Chemistry, 53: 836-844 (2015) https://dx.doi.org/10.1002/mrc.4281
Pushing The Limits Of Signal Resolution To Make Coupling Measurements Easier. Herbert Pucheta J.E., Pitoux, D., Grison, C.M., Robin, S., Merlet, D., Aitken, D.J., Giraud, N. & Farjon, J.* Chemical Communication, 51: 7939-7942 (2015) https://dx.doi.org/10.1039/c5cc01305d
Fully Resolved NMR Correlation Spectroscopy. Pitoux, D., Plainchont, B., Merlet, D., Hu, Z., Bonnaffé, D. Farjon, J., & Giraud, N.* Chemistry – A European Journal, 21: 9044-9047 (2015) https://dx.doi.org/10.1002/chem.201501182
Simultaneous Determination Of All Forms of Biopterin and Neopterin in Cerebrospinal Fluid. Guibal, P., Leveque, N., Doummar, D., Giraud, N., Roze, E., Rodriguez, D., Couderc, R., Billette De Villemeur, T. & Moussa, F.* ACS Chemical Neuroscience, 5: 533−541 (2014) https://dx.doi.org/10.1021/cn4001928
Exploration of Supramolecular Interactions Involving Tris-Dipicolinate Lanthanide Complexes in Protein Crystals By a Combined Biostructural, Theoretical and NMR Study . Dumont, E., Pompidor, G., D’Aléo, A., Vicat, J., Toupet, L., Maury, O. & Giraud, N.* Physical Chemistry Chemical Physics, 15 (41), 18235-18242 (2013) https://dx.doi.org/10.1039/C3CP53671H
A multidimensional approach to the analysis of NMR titration experiments in the case of a multiple reaction scheme. D’Aléo, A., Dumont, E., Maury, O. & Giraud, N.* Magnetic Resonance in Chemistry, 51 (10): 641–648 (2013) https://dx.doi.org/10.1002/mrc.3994
Combining J-edited and Correlation Spectroscopies Within a Multi-Dimensional Spatial Frequency Encoding: Toward Fully Resolved 1H NMR Spectra. Giraud, N., Pitoux, D., Ouvrard, J.M. & Merlet, D.* Chemistry –A European Journal, 19: 12221-12224 (2013) https://dx.doi.org/10.1002/chem.201302005
NMR in Organic Chemistry. Courtieu, J., Giraud, N., Lafon, O., Lesot, P., Lorthioir, C. & Nuzillard, J.M. Actualité Chimique, 364 : 30-41 (2012) http://www.lactualitechimique.org/La-RMN-en-chimie-organique
Spin-Spin Coupling Edition in Chiral Liquid Crystal NMR Solvent. Merlet, D., Beguin, L., Courtieu, J. & Giraud, N.* Journal of Magnetic Resonance, 209 (2): 315-322 (2011) https://dx.doi.org/10.1016/j.jmr.2011.01.030
Nuclear Magnetic Resonance Using a Spatial Frequency Encoding: Application to J-edited Spectroscopy Along the Sample. Giraud, N., Beguin, L., Courtieu, J. & Merlet, D.* Angewandte Chemie International Edition, 49 (20) :3481-3484 (2010) https://dx.doi.org/10.1002/anie.200907103
Improvements to Selective Refocusing phased (SERFph) experiments. Beguin, L., Giraud, N., Ouvrard, J.M., Courtieu, J. & Merlet, D.* Journal of Magnetic Resonance, 199 (1) : 41-47 (2009) https://dx.doi.org/10.1016/j.jmr.2009.03.012
Application of a 1H -Resolved 2D NMR Experiment to the Visualisation of Enantiomers in a Chiral Environment, using Sample Spatial Encoding and Selective Echoes. Giraud, N., Joos, M., Courtieu, J. & Merlet, D.* Magnetic Resonance in Chemistry, 47 (4) : 300-306 (2009) https://dx.doi.org/10.1002/mrc.2387
Protein Crystallography Through Supramolecular Interactions Between a Lanthanide Complex and Arginin Amino Acid. Pompidor, G., D’Aléo, A., Vicat, J., Toupet, L., Giraud, N.*, Kahn, R.* & Maury, O.* Angewandte Chemie International Edition 47 (18) : 3388-3391 (2008) https://dx.doi.org/10.1002/anie.200704683
The Role of 15N CSA and CSA/Dipole Cross-Correlation in 15N Relaxation in Solid Proteins. Sein, J., Giraud, N., Blackledge, M. & Emsley, L.* Journal of Magnetic Resonance 186 : 26-33 (2007) https://dx.doi.org/10.1016/j.jmr.2007.01.010
Solid-State NMR of a Paramagnetic Protein: Assignment and Study of Human Dimeric Oxidized Cu(II),Zn(II) Superoxide Dismutase (SOD). Pintacuda, G., Giraud, N., Pierattelli, R., Böckmann, A., Bertini, I. & Emsley, L.* Angewandte Chemie International Edition 46 (7) : 1079-1082 (2007) https://dx.doi.org/10.1002/anie.2006.03.093
The Influence of Nitrogen-15 Proton Driven Spin Diffusion on the Measurement of Nitrogen-15 Longitudinal Relaxation Times. Giraud, N., Blackledge, & Emsley, L.* Journal of Magnetic Resonance, 184 (1) : 51-61 (2006) https://dx.doi.org/10.1016/j.jmr.2006.09.015
Observation of Heteronuclear Overhauser Effects Confirms the 15N-1H Dipolar Relaxation Mechanism in a Crystalline Protein. Giraud, N., Sein, J., Pintacuda, G., Lesage, A., Böckmann, A., Blackledge, M. & Emsley, L.* Journal of the American Chemical Society, 128 (38) : 12398-12399 (2006) https://dx.doi.org/10.1021/ja064037g
Quantitative Analysis of Backbone Dynamics in a Crystalline Protein from Nitrogen-15 Spin-Lattice Relaxation. Giraud, N., Blackledge, M., Goldman, M., Böckmann, A., Lesage, A., Penin, F. & Emsley, L. * Journal of the American Chemical Society, 127 (51) : 18190-18201 (2005) https://dx.doi.org/10.1021/ja055182h
Site Specific Backbone Dynamics from a Crystalline Protein by Solid State NMR Spectroscopy. Giraud, N., Böckmann, A., Lesage, A., Penin, F., Blackledge, M. & Emsley, L.* Journal of the American Chemical Society126 (37) : 11422-11423 (2004). https://dx.doi.org/10.1021/ja046578g
Transverse Dephasing Optimized Solid State NMR Spectroscopy. De Paëpe, G. , Giraud, N., Lesage, A., Hodgkinson, P., Böckmann, A. & Emsley, L.* Journal of the American Chemical Society 125 (46) : 13938-13939 (2003). https://dx.doi.org/10.1021/ja037213j
Solid-state NMR sequential resonance assignments and conformational analysis of the 2×10.4 kDa dimeric form of the Bacillus Subtilis protein, Crh. Böckmann, A.*, Lange, A., Galinier, A., Luca, S., Giraud, N., Juy, M., Heise, H., Montserret, R., Penin, F., & Baldus, M. Journal of Biomolecular NMR 27 (4) : 323-339 (2003). https://dx.doi.org/10.1023/A:1025820611009