Our team is interested in the fragment-based design of RNA ligands and the development of methods to analyze RNA-binders interactions. This work is carried out in collaboration with the team of Dr. C. Tisné (Microbial Gene Expression Laboratory, UMR8261, IBPC) and the team of Dr. B. Limoges (Molecular Electrochemistry Laboratory, UMR 7591, University of Paris Diderot). Our current objective is to measure the interaction of an unlabeled molecule with a native RNA, in an aqueous buffer solution, using competition experiments with fluorinated probes (NMR detection) or redox probes (electrochemistry detection).
1. T. Lombès, R. Moumné, V. Larue, E. Prost, M. Catala, T. Lecourt, F. Dardel, L. Micouin, C. Tisné, Investigation of RNA–Ligand Interactions by 19F NMR Spectroscopy Using Fluorinated Probes. Angew. Chem. Int. Ed. 2012, 51, 9530-9534
2. H. Guyon, F. Mavré, M. Catala, S. Turcaud, F. Brachet, B. Limoges, C. Tisné, L. Micouin Use of a redox probe for an electrochemical RNA–ligand binding assay in microliter droplets Chem. Commun., 2017,53, 1140-1143